WebAug 25, 2016 · RNA extraction was carried out using TRIzon Reagent (CWBio, China) following the manufacturer’s instructions. Possibly contaminated genomic DNA was removed by DNase (Invitrogen, USA) treatment. The OD 260 :OD 280 ratio of total RNA was detected with Nanodrop 2000 (Thermo Scientific Inc., USA). WebMar 1, 2024 · After cell transfection, total RNA was extracted using Trizon reagent (CW0580, CWBIO, China) and reversely transcribed to cDNA using HiFiScript cDNA synthesis kit (CW2141, CWBIO, China) according to the manufacturer’s instructions.
Electrospun fiber membrane with asymmetric NO release for the ...
Web本发明公开了一种拟南芥长链非编码RNA‑DANA1及其在植物干旱耐受性的应用,涉及植物的基因工程领域。其中,所述的DANA1核苷酸序列如SEQ ID NO.1所示。本发明通过克隆拟南芥长链非编码RNA‑DANA1的全长转录本,并构建过表达载体获得DANA1过表达转基因植株,过表达DANA1后植物表现出失水速率减慢,对 ... WebSep 3, 2024 · To clarify the crucial differentially expressed genes (DEGs) induced by NNM in the TMV-infected plant cells, total RNA was extracted from NNM and mock treatment BY-2 protoplasts at 20 hpi with a TRIzon Reagent (CWBIO, Beijing, China) according to the manufacturer’s protocol. garmin nuvi 265w battery replacement
Decreased expression of m 6 A demethylase FTO in ovarian aging
WebSep 3, 2024 · Ningnanmycin (NNM) belongs to microbial pesticides that display comprehensive antiviral activity against plant viruses. NNM treatment has been shown to efficiently delay or suppress the disease... WebJan 19, 2024 · Many nitrate transporters, transcription factors, and protein kinases participate in the regulation of nitrate signaling. Here, we identified a gene encoding the chrysanthemum calcineurin B-like... WebNov 22, 2024 · Total RNA was extracted with Trizon Reagent (Cwbio, China). The first-strand cDNA was prepared using the HiFiScript cDNA Synthesis kit (Cwbio, China) according to … garmin nuvi 265w bluetooth