WebBCA™ Reagent A, 250 ml, contains sodium carbonate, sodium bicarbonate, bicinchoninic acid and sodium tartarate in 0.1 M sodium hydroxide BCA™ Reagent B, 25 ml, contains 4% cupric sulfate Compatibility Reagent, 10 × 20 mg Reconstitution Buffer, 15 ml Albumin Standard, 2 mg/ml, 10 × 1 ml ampules, contains bovine serum albumin at 2.0 mg/ml in Web• For samples containing > 0.0125% SDS, add one pack of Ionic Detergent Compatibility Reagent (IDCR, Product No. 22663) to 20mL of the Pierce 660nm Protein Assay …

Bio-rad protein assay reagent changes color in RIPA buffer?

WebThe Ionic Detergent Compatibility Reagent is intended for use with the 660 nm Protein Assay reagent (catalog numbers 22662 and 22660). It provides for even broader detergent compatibility, making the 660 nm Protein Assay one of the only protein … Webplease try out 660nM Pierce protein quantitation assay supplemented with a pack (or more as needed) ionic detergent compatibility reagent if you're using RIPA plus Triton-X100 lysis buffer. town of horseheads code enforcement

Pierce™ 660nm Protein Assay Reagent - Thermo Fisher …

Web88306 HiPPR Detergent Removal Spin Columns, 0.1mL, 24/pkg . 88307 HiPPR Detergent Removal 96-well Spin Plates, 0.1mL, 2 plates. 20308 SDS-Out SDS Precipitation Kit. 23225 Pierce BCA Protein Assay Kit. 22660 Pierce 660nm Protein Assay Reagent. 22663 Ionic Detergent Compatibility Reagent, 5 × 1g . Cited References 1. Rusconi, E., et al. (2001). WebMost surfactants cause precipitation of the reagent even at very low concentrations. One exception is sodium dodecyl sulfate (SDS), which is compatible with the reagent at … Web18 dec. 2024 · Since the protocol described here uses detergents during the homogenization step, ionic detergent compatibility reagent has to be added to the protein assay reagent. 6. Add 10 μL of each protein standard, such as bovine serum albumin (25, 50, 125, 250, 500, 750, 1,000, 1,500 and 2,000 μg/mL), the unknown samples and the … town of horseheads employment