Destabilization of medial meniscus
WebThis chapter describes the surgical procedure for destabilization of medial meniscus in mice. Details on subsequent microCT and histological analysis are also provided, as well as details on osteoarthritis evaluation. Related articles Based on techniques Generation and Characterization of Mouse Models for Skeletal Disease WebDesign: Acomys received destabilization of the medial meniscus (DMM) surgery (n = 11) or a skin incision (n = 10). Gait testing occurred at 4, 6, 8, 10, and 12 weeks after surgery. At endpoint, joints were processed for histology to assess cartilage damage. Results: Following joint injury, Acomys with DMM surgery altered their walking patterns ...
Destabilization of medial meniscus
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WebNov 11, 2024 · Mice subjected to surgery for destabilization of the medial meniscus (OA group) or control surgery (Control group) were sacrificed after eight weeks. The knees were collected for histological assessment. WebThe surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of posttraumatic osteoarthritis (OA).
WebExperimental OA was induced in 12-week-old male mice (C57BL/6J) by destabilization of the medial meniscus (DMM) surgery as described by Glasson et al. . The medial meniscus tibial ligament was exposed without sectioning in sham surgery. Mice were sacrificed 8 weeks after DMM or sham surgery and subjected to histological analyses. WebJun 28, 2024 · A mouse OA model was induced using destabilization of the medial meniscus (DMM) surgery, chondrocyte ferroptosis was induced using an intra-articular injection of Erastin, and Met (200 mg/kg/day) was intragastrically administered for 8 weeks after surgery. H&E and Safranin O‑fast green staining were used to evaluate cartilage …
WebApr 6, 2024 · Tryptase β regulation of joint lubrication and inflammation via proteoglycan-4 in osteoarthritis - PMC Back to Top Skip to main content An official website of the United States government Here's how you know The .gov means it’s official. Federal government websites often end in .gov or .mil. WebSep 29, 2024 · Destabilization of the medial meniscus (DMM) is commonly used as a surgical model of OA in the mouse . In this model transection of the mediomeniscotibial ligament (MMTL) results in a slowly progressing …
WebDestabilization of medial meniscus (DMM) model is an important tool for studying the pathophysiological roles of numerous arthritis associated molecules in the pathogenesis of osteoarthritis (OA) in vivo. However, the detailed, especially the visualized protocol for establishing this complicated model in mice, is not available.
WebFeb 10, 2024 · We further investigated whether kindlin-2 loss impacts progression of a destabilization of the medial meniscus (DMM)-induced OA model. At 2 months of age, the K2fl/fl; AggrecanCreERT2 mice were... flying with a hematomaWebJan 1, 2014 · The surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of posttraumatic osteoarthritis (OA). The DMM model … flying with a guitar on american airlinesWebAbstract: Destabilization of medial meniscus (DMM) surgical instability models of osteoarthritis (OA) have been underutilized despite having many inherent advantages. At present, there is a lack of studies clarifying cartilage degenerative changes induced by DMM in rats, which would further verify a greater use of this model. Therefore, green mountain leatherWebdifferences in a destabilized medial meniscus osteoarthritis model. Methods: Male and Female mice receive either a destabilized medial meniscus or sham procedure resulting … green mountain ledge grill for saleWebFeb 9, 2024 · Age is the primary risk factor for osteoarthritis (OA), yet surgical OA mouse models such as destabilization of the medial meniscus (DMM) used for evaluating … flying with a guitarWebOsteoarthritis Mouse Model of Destabilization of the Medial Meniscus This chapter describes the surgical procedure for destabilization of medial meniscus in mice. Details on subsequent microCT and histological analysis are also provided, as well as details on osteoarthritis evaluation. green mountain lawyers vermontWebNational Center for Biotechnology Information flying with a gun on american airlines